Group H-3

　　　　　　　　　　　　　　　　　　　　　　　　　　　　

Group H-3 化学構造　(Chemical Structure)

● Hibiscus manihot powder
　CAS:　　　 Food/Natural MW:

AM Sal Max (?) □ 1)

CA CHL/IU Max ( 1.0 mg/ml, -S9), 24, 48h　(No data for +S9) □ 2)

1) Natl. Inst. Hygien. Sci., Tokyo, Japan (?)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998)

●Hinokitiol 　(ヒノキチオール）
　　 499-44-5 　Food　 164.20

CA CHL/IU Min( 0.002 mg/ml, -S9), 24, 48h; D20=0.0014; TR=1000 ◎ 1)

1) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998) (Tables in English)

● L-Histidine monohydrochloride
　　　71-00-1　Food　　 209.63

AM Sal Max (?) □ 1)

CA CHL/IU Max ( 2.0 mg/ml, -S9), 24, 48h　(No data for +S9) □ 2)

1) Natl. Inst. Hygien. Sci., Tokyo, Japan (?)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998) (Tables in English)

● Hop oil English Sas
　　　Food/Natural

CA CHL/IU Max ( 0.125 mg/ml, -S9), 24, 48h　(No data for +S9) □ 1)

1) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998)　(Tables in English)

● Hydantoic acid 　（ヒダントイン酸）
　　462-60-2　　 118.09

CA CHL/IU Max ( 1.0 mg/ml, -S9), 48h　(No data for +S9) △ 1)

1) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998)　(Tables in English)

●Hydralazine-HCl　（ヒドララジン塩酸塩）
　　　　304-20-1　Medicine 　 196.64

AM Sal Min ( 0.6 mg/ml, -S9) ○ 1)

CA CHL/IU Min ( 0.004 mg/ml, -S9), 24, 48h　(No data for +S9)；
D20= 0.004; TR= 530 ◎ 2)

1) De Flora et al., Environ. Mol. Mutagen., 4, 605-619 (1982)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998)　(Tables in English)

【Note】 (Cited from IARC Monographs, Suppl.., 6 (1987)

　　No data were available on the genetic and related effects of hydralazine in humans.
　　In a single, limited study, hydralazine did not induce DNA damage in animals treated invivo. It induced SCEs in human lymphocytes in vitro, whereas assays for CAs in rodent cells in vitro were inconclusive. Hydralazine induced UDS in rat and rabbit hepatocytes in vitro and induced mutaion and DNA damage in bacteria. . (IARC Monographs, 24, 85)

●Hydrazine (anhydrous)　（ヒドラジン（無水））
　　　　302-01-2　Medicine 　 32.05

MB B. subilis Min (?) ○ 1, 2)

AM Sal. Min ( 0.01 mg/ml, -S9) ○ 17)

AM Sal/E. coli Min ( 2.24 μg/plate, +S9); 　spa= 52100 ◎ 18, )

AM Sal. Min ( 1.0 mg/plate, +S9); ○ 19, 20)

AM E. coli Min ( 0.36 mg/plate, ±S9);

PLN Rice Min (?) ○ 3)

SPL Drosophila Min (?) ○ 4)

SM CHO/HGPRT Min (?) ○ 5)

MLA L5178Y Min ( 0.16 mg/plate)　 ○ 6)

SM Rat-liver
epitherial cells Min ( 0.04 mg/plate) (H-ras gene) ○ 7)

HMA Mice Min (?) ○ 8)

SLRL Drosophila Min (?) ○ 9)

CAv C. faba Min (?) ○ 10)

CA CHO Min (?) ○ 11)

CA CHL/IU Min ( 0.1 mg/ml, -S9); D20= 0.074 ◎ 18)

SCE CHO Min (?) ○ 12, 13)

MN V79 Min ( 0.003 mg/plate, -S9) ○ 21)

CT Hum. Fb Min ( 0.035 mg/pate, -S9) ○ 22)

MNv Mice Min (?) ○ 14)

MNv Mice Max (?) □ 15)

DLv Mice Max (?)　(ip) □ 16)

1) Freese EB, et al,: Mutation Res, 4, 517-531 (1967)
2) Bresier SE, et al,: Mutation Res., 5, 1-14 (1968)
3) Reddy GM & Reddy TP: Ind. J. Genet. Plant Breed., 32, 388-391 (1972)
4) Jain HK & Shukla PT: Mutation Res., 14, 440-442 (1972)
5) Hsie AW, et al,: In FJ de Serres & J Ashby (Eds); Prog. Mutation Res., 1, 602-607 (1981)
6) Rogers AM & Back KC: Mutation Res., 89, 321-328 (1981)
7) Kumari HL, et al,: Proc. Am. Assoc. Cancer Res., 33, 192 (1992)
8) Rohrborn G, et al,: Mutation Res., 16, 189-194 (1972)
9) Shukla PT: Mutation Res., 16, 363-371 (1972)
10) Gupta RS & Grover NS: Mutation Res., 10, 519-520 (1970)
11) Natarajan AT, et al,:In FJ de Serres & J Ashby (Eds); Prog. Mutation Res., 1, 551-559 (1981)
12) MacRae WD & Stich HF: Mutation Res.68, 351-365 (1979)
13) Spelt G, et al,: Hum. Genet., 54, 155-158 (1980)
14) Salomone MF, et al,: In FJ de Serres & J Ashby (Eds); Prog. Mutation Res., 1, 686-697 (1981)
15) Tsuchimoto T & Matter BE: In FJ de Serres & J Ashby (Eds); Prog. Mutation Res., 1, 705-71 (1981)
16) Epstein SS, et al,: toxicol. Appl. Pharmacol, 23, 288-325 (1972)
17) Anderson D & Styles JA: Bri. J. Cancer, 37, 924-930 (1978)
18) Ministry of Labour, Japan, Mutagenicity Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996) (Tables in English)
19) Herbold B & Buselmaier W., Mutation Res., 40, 73-84 (1976)
20) McMahon RE., et al., Cancer Res., 39, 682-693 (1979)
21) Onfelt A., Mutation Res., 113, 357391 (1987)
22) Mil GE,m et al., In Vitro, 17, 719-729 (1981)

【Note】 (Cited from IARC Monographs, Suppl.., 6 (1987)

　　No data were available on the genetic and related effects of hydrazine in humans.
　　Hydrazine did not induce DL mutation or MNs in bone-marrow cells of mice treated in vivo. It induced UDS in human cells in vitro. It did not induce CAs in rat cells in vitro but induced SCEs in Chinese hamster cells; conflicting results were obtained for the induction of mutation in mouse lymphoma cells. It induced DNA strand breaks in rat hepatocytes in vitro. Hydrazine induced somatic mutation in Drosophila and CAs and mutation in plants. It was mutagenic to yeast and bacteria and induced DNA damage in bacteria. (IARC Monographs, 4, 127)

● 2-Hydrazinoethanol
　CAS：109-84-2　Industry 　MW: 76.10

AM Sal/ E.coli Min (26.3 μmol, -S9)
○ 1)

1) Moriya M., et al., Mutation Res., 57, 259 (1978)

● Hydrochlorothiazide
　　　　CAS：58-93-5　Medicine 　MW: 297.75

AM Sal Max (?) □ 1)

CA CHL/IU Max ( 0.5 mg/ml, -S9), 24, 48h (No data for +S9)；
Also poly, 48h △ 2)

1) Natl. Inst. Hygien. Sci., Tokyo, Japan (?)
2) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test In Vitro LIC, Tokyo (1998)　(Tables in English)

●Top Page　（トップページ）

●Abbreviation 　（省略記号）　

●Mutagenicity 　（変異原性）

●Test Systems　(試験法の種類）

●Technical Problems　（技術的問題点）

●List of　Compounds（化合物リスト）

●Evaluation of Results　（試験結果の評価）

AM	Sal	Max (?)	□	1)
CA	CHL/IU	Max ( 1.0 mg/ml, -S9), 24, 48h　(No data for +S9)	□	2)

AM	Sal	Min ( 0.6 mg/ml, -S9)	○	1)
CA	CHL/IU	Min ( 0.004 mg/ml, -S9), 24, 48h　(No data for +S9)； D20= 0.004; TR= 530	◎	2)

MB	B. subilis	Min (?)	○	1, 2)
AM	Sal.	Min ( 0.01 mg/ml, -S9)	○	17)
AM	Sal/E. coli	Min ( 2.24 μg/plate, +S9); 　spa= 52100	◎	18, )
AM	Sal.	Min ( 1.0 mg/plate, +S9);	○	19, 20)
AM	E. coli	Min ( 0.36 mg/plate, ±S9);
PLN	Rice	Min (?)	○	3)
SPL	Drosophila	Min (?)	○	4)
SM	CHO/HGPRT	Min (?)	○	5)
MLA	L5178Y	Min ( 0.16 mg/plate)	○	6)
SM	Rat-liver epitherial cells	Min ( 0.04 mg/plate) (H-ras gene)	○	7)
HMA	Mice	Min (?)	○	8)
SLRL	Drosophila	Min (?)	○	9)
CAv	C. faba	Min (?)	○	10)
CA	CHO	Min (?)	○	11)
CA	CHL/IU	Min ( 0.1 mg/ml, -S9); D20= 0.074	◎	18)
SCE	CHO	Min (?)	○	12, 13)
MN	V79	Min ( 0.003 mg/plate, -S9)	○	21)
CT	Hum. Fb	Min ( 0.035 mg/pate, -S9)	○	22)
MNv	Mice	Min (?)	○	14)
MNv	Mice	Max (?)	□	15)
DLv	Mice	Max (?)　(ip)	□	16)