REC	B.subtilis	Max ( 5 mg/disk, )	□	1)
AM	Sal./E.coli	Max （0.01-1.0 mg/plate, ±S9) :	□	1)
CA	CHL/IU	Max ( 1 x 10-4 M, ±S9)	□	1)

1) DuPont Company:, Japan : J. Pesticide Sci., 16, 343-347 (1991)

● Bensultap (ベンサルタップ）
　　17606-31-4　　Pesticide 　431.63

REC	B.subtilis	Max (10 mg/disk, )	□	1)
AM	Sal./E.coli	Max （5.0 mg/plate, ±S9) :	□	1)
MNv	Mice/C3H	Max ( 100 mg/kg x 5)	□	1)
DLv	(?)	Max (?)	□	1)
UDS	(?)	Max (?)	□	1)

1) Takeda Pharm. Co. Ltd: Jap. J. Pesticide Sci., 14, 523-529 (1989)

● Benthiazole (カビサイド；　ブーサン）
    21564-17-0　Pesticide　　238.35

REC	B.subtilis	Min ( 0.4 mg/well, -S9)	△	1)
AM	Sal.	Min (?)	○	1)

1) Sankyo Chem. & Ohtsuka Chem. Co Ltd.: Jap. J. Pesticide Sci., 12, 343-345 (1987)

● Benz [a] anthracene （ベンツアントラセン）（B [a] A）
　　56-55-3　　Laboraory/Carcinogen　　228.3

AM	Sal.	Max (?)	□	1)
AM	Sal.	Min (?) +S9	○	2)
YM	Yeast	Max (?)	□	3)
SM	V79 (6-TG)	Min (109 nmol/ml, +S9)	○	4)
UDS	Human Fb	Max (?)	□	5)
UDS	HeLa Cells	Min (10-7 M, +S9)	○	6)
UDS	Rat Hepatocytes	Max (1μg/ml)	□	7)
CT	Rat Embryo Cells	Min (?)	○	8)
CT	SH Cells	Min (10 μg/ml)	○	9)

1) Brown JP. & Brown RJ.: Mutation Res., 40, 203-224 (1976)
2) McCann J., et al.: Proc. Natl Acad. Sci.(USA), 72, 5135-5139 (1975)
3) Murthy MSS.: Mutation Res., 64, 1-17 (1979)
4) Krahn DF. & Heidelberger C.: Mutation Res. 46, 27-44 (1977)
5) San RHC. & Stich HF.: Int. J. Camcer,16, 284-291 (1975)
6) Martin CN., et al.: Cancer Res., 38, 2621-2627 (1978)
7) Williams GM.: Cancer Res., 37, 1845-1851 (1977)
8) Freeman AE. et al.: J. Natl Cancer Inst., 51, 799 (1973)
9) DiPaolo JA., et al.: J. Natl Cancer Inst., 42, 867-876 (1969)

● Benzal chloride (ベンザルクロリド; ベンジリデンジクロリド）
　　98-87-3　　Industry　　161.03

DNA	Rec-assey	Min.( 5 mg/ml, -S9)	○	1)
AM	Sal.(TA100)	Min ( 0.01 mg/ml, +S9),	○	1,2)
AM	E.coli	Min ( 0.01mg/ml, +S9)	○	1)

1) Yasuo, K., et al., ; Mutation Res., 58, 143-150 (1978)
2) BUA Reportt, 71 (1994)
3) Hazardous Substances Data Bank (HSDB), U>S> Natl Library of Medicine (1998)

● Benzaldehyde （ベンツアルデヒド） (Benzoic acid aldehyde)
　　100-52-7　　Industry　　106.12　

AM	Sal./E.coli	Max ( 1.25 mg/plate, ±S9)	□	1)
AM	Sal.	Max (?), ±S9)	□	6)
AM	Sal.	Max ( 1 mg/plate, ±S9)	□	7)
AM	Sal.	Max ( 0.5 mg/plate, ±S9)	□	3)
AM	Sal.	Max ( 2 mg/plate, ±S9)	□	8)
AM	Sal.	Max ( 3.3 mg/plate, ±S9)	□	9)
MLA	L5178Y	Min ( 0.8 mg/ml, -S9)	○	10)
CA	CHL/IU	Min ( 1.2 mg/ml, -S9), 6-18h; D20=0.99; TR=18 Min ( 0.8 mg/ml, ±MS9), Poly	○	2)
CA	CHL/IU	Min ( 0.45 mg/ml, -S9),24h; D20=0.99; also Poly	○	5)
CA	CH, B241	Min ( 0.005μg/ml, ±S9), 24h	○	3)
CA	CHO	Max (1.6 mg/plate, ±S9)	□	11)
CA	Vicia faba (ソラマメ)	Max (?)	□	4)
SCE	CHO	Min ( 0.16 mg/ml, ±S9)	○	12)
SCE	Hum. lym	Min (106 mg/L, ±S9)	○	13)
SLRLv	Drosophila	Max (2500 ppm) fed	□	14)

1) Ministry of Labor, Japan; Mutagen. Test Data of Exist. Chem., JETOC (Ed.), Suppl. 2 (2000)
2) Sofuni T. (Ed.): Data Book of Chromosome Aberration Test in vitro, LIC, Tokyo (1998） (Tables in English)
3) Kasamaki A, et al: Mutation Res., 105, 387-392 (1982)
4) Auerbach C, et al : Mutation Res., 39, 317-362 (1977)
5) Ministry of Labor, Japan; Mutagen. Test Data of Exist. Chem., JETOC (Ed.), Suppl. 3, pp.255 (2005) (Tables in English)
6) Florin I., et al., Toxicol., 18,219-232 (1980)
7) Haworth S., et al., Environ. Mutagen., 5 (Suppl 1) 3-142 (1983)
8) Nohmi T., et al., Bull Natl Inst., Hyg., Sci., 103, 60-64 (1985)
9) Dillon D., et al., Mutagenesis, 13, 19-26 (1998)
10) McGregor DB., et al., Environ. Mol. Mutagen., US-NTP Rep. (1990)
11) Galloway CM., et al., Environ. Mol. Mutagen., 10 (Suppl. 10), 1-175 (1987)
12) Matshuoka A., et al., Environ. Mol. Mutagen., 20, 159-165 (1998)
13) Jensson T., et al., Mutation Res., 206, 17-24 (1988)
14) Woodruff RC., et al., Environ. Mutagen., 7, 677-702 (1985)

IARC Carcinogenicity Criteria：　Group 1

● Benzene 　（ベンゼン）
　　71-43-2　Industry/Solvent　78.11

AM	Sal.	Max (?), (+S9)	□	1)-4)
AM	Sal.	Min (?)	○	5)
YM	Yeast	(Mitotic Crossing Over) Max (?)	□	6)
YM	Yeast (D61. D6)	Min (?) (Gene Conversion Test)	○	7)
SM	Drosophila (Eye)	Max ( 2.5%), fed	□	8)
SM	Mammalian Cells	Max ( 3.0 mg/ml, ±S9	△	9)
CA	Human LY	Min ( 88μg/ml, ±S9), 3h	○	10)
CA	CHL/IU	Min ( 1.1 mg/ml, +S9 ), 6-18h ; D20=2.6; TR=9.1	○	11)
CA	CHO	Max ( 937 μｇ／ｍｌ, +S9)	□	12)
CA	Primary Hepatocytes	Min ( 62.5 μg/ml)	○	13)
SCE	Human LY	Max ( 1.52 mg/ml)	□	14)
SCEv	Mouse/BM	Min ( 28 ppm), ih, 4h	○	15)
UDS	Rat/HP	Min ( 10-3 M)	○	16)
DNA	L5178Y	Single-strand breaks, Max ( 1.0 mmol)	□	17)
CAv	Rat/ BM	Min ( 100 or 1000 ppm), ih	○	18)
MNv	Mouse/BM	Min ( 10-400 ppm, ih), 6h/day	○	19)
MNv	Mouse/PB	Min ( 25 mg/kg/d), 120 days	○	20)
SCEv	Mice	Min ( 10 ppm), 6h, ih	○	21)
CT	Hamaster (SHE)	Min ( 20 μg/ml, -S9)	○	22)
MLA	L5178Y	Max ( 1 mg/ml, ±S9)	□	23)

1) Dean BJ.: Mutation Res., 154, 153-181 (1985)
2) Lebowitz H., et al.: Mutation Res., 154,153-181 (1985)
3) Bartsch H., et al.: Mutation Res., 76, 1-50 (1980)
4) Shimizu M., et al.: Mutation Res.,116, 217-238 (1983)
5) McCarroll NE., et al.: Environ. Mutagen. 2, 281-282 (1980)
6) Parry JM.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp. 25-46, J. Ashby, et al　(Eds),, Elsevier, Amsterdam (1985)
7) Parry JM.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp. 261-269, J. Ashby, et al　(Eds), Elsevier, Amsterdam (1985)
8) Nylander PO., et al.: Mutation Res., 163-167 (1978)
9) Garner RC.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp. 85-94, J. Ashby, et al.(Eds),, Elsevier, Amsterdam (1985)　
10)Howard CA., et al.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp.457-467 J.Ashby, et al.(Eds),, Elsevier, Amsterdam　(1985)
11) Ishidate MJr.(Ed.): Data Book, Chromosomal Aberration Test in vitro LIC, Tokyo/Elsevier, Amsterdam (1988)
12) Natarajan, AT, et al; Prog. Mutation Res., 5, 433-437 (1985)
13) Danford ND.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp.397-411, J. Ashby, et al. (Eds),, Elsevier, Amsterdam　(1985)
14) Gerner-Smidt, P & Friedrich, U; Mutation Res., 58, 313-316 (1978)
15) Tice RR., et al.: Environ. Mutagen. 3, 338-339 (1981)
16) Glauert HP., et al. Reprort of the IPCS Collaborative Study on In Vitro Assays, pp.371-373　J.Ashby, et al.(Eds), Elsevier, Amsterdam (1985)
17) Pellack-Walker P. & Blumer JL.: Molec. Pharmacol., 30, 42-47 (1983)
18) Styles JA. & Richardson CR.: Mutation Res., 135, 203-209 (1984)
19) Tice RR.,, et al.: Environ. Mutagen. 6, 421 (1984)
20) Choy WN., et al.: Mutation Res., 143, 55-59 (1985)
21) Erexson GL., et al.: Environ. Mutagen. 8,29-40 (1986)
22) McGregor D. & Ashby J.: Reprort of the IPCS Collaborative Study on In Vitro Assays, pp.103-115, J. Ashby, et al.(Eds),, Elsevier,　 Amsterdam (1985)
22) Oberly TJ., et al., Mutation Res., 125, 291-306 (1984)

US-NTP Genotoxicity Screening:
○ Ames Test: □
○ CA　Test: 　□
○ SCE Test: ○
○ Drosophila (SLRL) Test: □
○ MLA Test: 　□

【Note】 (Cited from (IARC Monographs, Suppl., 6, 1987)

CAs in human peripheral lymphocytes have been associated with occupational exposure to benzene, although many of the studies are very difficult to interpret. 　Benzene induced CAs, MNs and SCEs in bone-marrow cells of mice, CAs in bone-marrow cells of rats and CH and sperm-head anomalies in mice treated in vivo. It induced CAs and mutation in human cells in vitro but did not induce SCEs in cultured human lymphocytes, except in one study in which high concentration of an exogenous metabolic system were used. In some test systems, it induced CT. It did not induce SCEs in rodent cells in vitro, but did induce aneuploidy and, in some studies, CAs in cultured CHO cells. It induced mutation and DNA damage in some studies in rodent cells in vitro. 　In Drosophila, it was reported to be weakly positive in assays for somatic mutation and for crossing-over in spermatogonia; in single studies, it did not induce SLRL mutation or translocations. It induced aneuploidy, mutation and gene conversion in fungi. it was not mutagenic to bacteria. (IARC Monographs, 7, 203, 1974)

AM

Sal./E.coli

Max ( 5.0 mg/plate,±S9)

□

1)

1) Ministry of Labor, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) (1996) (Tables in English)

● 1,3-Benzenedicarbonitrile (isophthalonitrile)
     626-17-5  Industry   128.13     

AM

Sal./E.coli

Max ( 5.0 mg/plate,±S9)

□

1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) Suppl. 2 (2000) (Tables in English)

● α-Benzenehexachloride （α-BHC）
　　319-84-6 　Industry　 290.83

CA

CHL/IU

Max ( 0.8 mg/ml, ±S9), 6-18h

□

1)

1) Sofuni T. (Ed.): Data Book of Chromosomal Aberration Test in vitro, LIC, Tokyo (1998）(Tables in English)

● γ-Benzenehexachloride (γ-BHC)
　　 58-89-9　　Industry 　　290.83

AM	Sal./E.coli	Max (?)	□	1)
CA	CHL/IU	Max ( 0.2 mg/ml, ±S9), 6-18h	□	2)
MN	Mice	Max (?)	□	3)

1) Bull Natl Inst., Hyg., Sci. (?)
2) Sofuni T. (Ed.): Data Book of Chromosome Aberration Test in vitro, LIC, Tokyo (1998） (Tables in English)
3) (?)

● 1,2,4-Benzenetricarboxylic acid 1,2-anhydride (Trimellianhydride)
　　 552-30-7　Industry 　　192.13

AM

Sal./E.coli

(±S9) :

□

1)

1) Ministry of Labour, Japan; Mutagen. Test Data of Exist. Chem., JETOC (Ed.), Suppl. 2 (2000) (Tables in English)

● Benzidine （ベンジディン）　　　　　　　　　　　　　　　　　　　　　　　　　　
　　 92-87-5　　　Industry　　184.23　　　

AM	Sal.(TA98)	Min ( 2.8 μg/mｌ, +S9)	○	1)
AM	E.coli	Max ( 2mg/mｌ, ±S9)	□	2)
YM	Gene conv.	Min ( 0.1 mg/ml, -S9)	○	3)
YM	Pombe	Max ( 2.5μg/mｌ, ±S9)	□	4)
PLM	Tradescantia	Min ( 0.1μg/mｌ, -S9)	○	5)
SLRLv	Drosophila	Min ( 3.6mg/kg)	○	6)
UDS	Rat liver cells	Min (1.8μg/mｌ, -S9)	○	7)
UDS	HeLa Cells	Min (10-7 M, +S9)	○	8)
SM	CHO	Min ( 20 μg/mｌ, ±S9)	○	9)
SCE	Ch	Min ( 0.1 mg/ml, +S9)	○	10)
CA	Rat cells	Min ( 12.5 μg/mｌ, -S9)	○	11)
CT	Balb C3T3	Min ( 1.0 μg/mｌ, -S9)	○	12)
CT	BHK 21Cells	Min ( +S9)	○	13)
UDSv	Rat liver	Min ( 0.2 mg/kg)	○	14)
SCEv	Rat liver	Min ( 7.7 mg/kg)	○	15)
MNv	Rat	Min ( 93 mg/kg)	○	16)
DNA	Binding	Min ( 11.6 mg/kg)	○	17)

1) Robertson, IGC, et al.; Cancer Res., 43, 476-480 (1983)
2) Matsushima, T. et al., Mutation Res., 1, 387-395 (1981)
3) Sharp DC & Parry, JM; Prog. Mutation Res., 1, 502-516 (1981)
4) Loprieno N., Prog. Mutation Res., 1, 424-433 (1981)
5) Van't Hof, J & Schairer, LA; Mutation Res., 99, 303-315 (1982
6) Fahmy, MJ & Fahmy, OG,; Mutation Res., 56, 31-38 (1977)
7) Willliams, GM; Cancer Lett., 4, 69-75 (1978)
8) Martine CN., et al.: Cancer Res., 38, 2621-26 27 (1978)
9) Gupta, RS & Singh, B; Mutation Res., 94, 449-466 (1982)
10) Evans, EL & Mitchell, AD; Mutation Res., 1,538-550 (1981)
11) Dean, BJ; Prog. Mutation Res., 1, 570-579 (1981)
12) Cortesi, E et al., Teratog. Carcinog. Mutagen., 3, 101-110 (1983)
13) Styles, JA; Brit. J. Cancer, 37, 931-936 (1978)
14) Mirsalts, JC et al., Environ. Mol. Mutagen., 4, 553-562 (1982)
15) Parodi、S., et al., Mutation Res., 108, 225-238 (1983)
16) Salamone et al., 686 1981
17) Martin et al., 2678 1982

 US-NTP Genotoxicity Screening:
○ Ames Test: ○
○ CA　Test: 　○
○ SCE Test: 　○
○ MLA　Test: 　○
　
 IARC Carcinogenicity Criteria:  Group 1

【Note】 (Cited from IARC Monographs, Suppl., 6, 1987)　

No data were available on the genetic and related effects of benzidine in humans. 　Convalent binding products of benzidine with DNA have been described in the liver of mice and rats treated in vivo. It induced MNs, SCEs, DNA strand breaks and UDS in cells of rodents treated in vivo. It induced UDS in human cells in vitro. It caused CT of Syrian. hamster embryo and BALB/c 3T3 cells and induced CAs, SCEs, UDS and DNA strand breaks in rodent cells in vitro; conflicting results were obtained for mutation. It induced aneuploidy, gene conversion and DNA damage in yeast, but not mutation. It was mutagenic to plants and bacteria. (IARC Monographs, 1, 80, 1972; 29, 149, 391, 1982)

AM	Sal./E.coli	Min  (0.004 mg/plate, ±S9), Spa=27700 (TA100, -S9)	◎	1)
CA	CHL/IU	Min (0.0004 mg/ml, -S9), 24h; D20=0.0005; TR=28000	◎	2)

 1) Ministry of Labour, Japan; Japan Bioassay Res. Center, Ann. Rep. (2005);  Ministry of Labor, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) Suppl.4, (2008), p.135 (Tables in English)
 2) Ministry of Labour, Japan; Japan Bioassay Res. Center, Ann. Rep. (2005); Ministry of Labor, Japan; Mutagen. Test Data of Exist. Chem. Subst., JETOC (Ed.) Suppl.4, (2008), p.182 (Tables in English)

●Top Page　（トップページ）

●Abbreviation 　（省略記号）

●Mutagenicity 　（変異原性）

●Test Systems　(試験法の種類）

●Technical Problems　（技術的問題点）

●List of　Compounds（化合物リスト）

●Evaluation of Results　（試験結果の評価）